Chemical Analysis and Preservation
To determine if the structures were biological in origin rather than contamination, the team used high-performance liquid chromatography–mass spectrometry. This analysis revealed that the bone extract was dominated by heme B, a molecule that helps blood cells carry oxygen. The researchers also detected traces of hydroxyproline, an amino acid associated with collagen.
To rule out contamination from the surrounding environment, the team compared the bone's chemistry to the surrounding rock. While the surrounding rock contained signatures of photosynthetic microbes, such as chlorophyll and bacteriochlorophyll, the heme B was found specifically within the bone extract.
The researchers suggest the exceptional preservation may be due to the geological setting: a microbial mat in shallow, low-oxygen waters. They propose that manganese oxide mineralization, specifically a mineral called hausmannite, may have helped stabilize or replicate the delicate cellular structures during fossilization.